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The concentration of GSH can be determined either as an endpoint read of the color developed at 405nm after 30 minutes or by measuring the rate of color development at 405nm

I do not give out my ever-changing protocol because it is highly personalized

After sample denaturation and electrophoresis, membranes were blocked with 5% milk in TBS-T (120 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 0.05% Tween 20) for 1 h at room temperature

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